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主营:分子类,蛋白类,抗体类,生化类试剂
℡ 4000-520-616
℡ 4000-520-616
BioBasic/Extraction Buffer for 2D Gel with Diluent, 50ml Urea, Thiourea, CHAPS and ASB-16/50ml/PL040
产品编号:PL040
市  场 价:¥1732.60
场      地:美国(厂家直采)
产品分类: 蛋白类>聚合物>
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$86.63
品      牌: BioBasic
公      司:BioBasic
公司分类:
BioBasic/Extraction Buffer for 2D Gel with Diluent, 50ml Urea, Thiourea, CHAPS and ASB-16/50ml/PL040
商品介绍
Product Description:Extraction Buffer for 2D Gel with Diluent, 50ml Urea, Thiourea, CHAPS and ASB-16: 2D buffers must solubilize proteins effectively without disturbing the native charge on the proteins. Urea, a common chaotrope, is widely used for solubilization and denaturation of proteins. One of the disadvantages of using urea is carbamylation. Urea in water exists in equilibrium with ammonium cyanate, of whose level increases with increasing temperature and pH. Cyanate reacts with α-amino and ε-amino groups of proteins and induces a change in the isoelectric point. This leads to artifacts in results and therefore carbamylation must be avoided. One way to minimize the risk of carbamylation is to prepare the urea based reagents fresh before each use. Bio Basic Inc. has developed a series of dry urea based pre-mixed and ready-to-use solubilization buffers. Simply add an appropriate volume of the supplied diluent buffer to the dry powder and then use directly to solubilize proteins, saving time and improving the quality of IEF/2D gel electrophoresis. 2D Sample Extraction Buffers are also designed to be used as rehydration buffers for IPG strips. 2D Sample Extraction Buffers are experimentally optimized with proprietary technologies and critical buffering and stabilizing agents, including urea, thiourea, Nonidet® P-40, CHAPS, and sulfobetaines. Our 2D Sample Extraction Buffers are designed to produce optimal protein extraction and significantly improved spot resolution for 2D gel analysis. A range of 2D Sample Extraction Buffers have been developed and depending on the nature of the samples, one or more of the buffers suitable for your applications can be ordered. PL037 is suitable for most applications, however for stronger solubilization effects, we recommend PL038-PL042.
Total Product Size:50ml
Individual Container Size:50ml
Number of Containers:1
Refrigeration Requirements:Freezer
Shipping Conditions:ICE
UNSPSC Code:41105323
UNSPSC Category:Electrophoresis Buffers
品牌介绍

Bio Basic Inc.為加拿大知名化學藥品供應商,Bio Basic公司提供各式生物實驗用化學藥品、生物製劑,藥品種類豐富且選擇多樣,提供最經濟實惠的價格,為生物實驗用化學藥品的第一選擇。
 
盟基生物科技為加拿大Bio Basic在台灣經銷商,盟基生物科技的專業服務團隊提供了研究人員完整的銷售前與銷售後的技術資源,讓您於研究上無後顧之憂。

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